| 订购货号 | 产品名称及规格 | 价格（￥） |
| 20010 | dsGreen Gel Staining Solution, 10000×, 1 mL | 2340.00 |
SYBR Green I是一种敏感的dsDNA结合染料，可以用于日常琼脂糖和聚丙烯酰胺凝胶的DNA检测。
不同于Ethidium，SYBR Green I对双链DNA有高度选择性，更低的毒性，更高的灵敏度。
溴化乙锭（Ethidium Bromide）和SYBR Green I的对比
|
Feature |
Ethidium Bromide |
SYBR Green I |
|
Fluorescence |
Red (615 nm) |
Green (524 nm) |
|
Excitation maximum |
302 nm |
454 nm |
|
Excitation light source |
UV only |
Blue light or UV |
|
Sensitivity |
2 ng / band(dsDNA) |
0.08 ng / band (dsDNA) |
|
Health hazard |
High |
Low |
推荐手册：
SYBR Green I DNA凝胶染色
Note: fluorescent properties of SYBR Green I bound to dsDNA below are taken from publication: Zipper, H.; Brunner, H.; Bernhagen, J.; Vitzthum, F. Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications. Nucleic Acids Res., 2004, 32, e103.
General properties
|
Appearance: |
orange solution |
|
Quality control: |
NMR 1H (95%) and 13C, TLC, functional testing |
|
Storage conditions: |
Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
|
MSDS: |
Download |
Spectral properties
|
Excitation maximum, nm: |
454 |
|
Extinction coefficient at excitation maximum, Lmol-1cm-1: |
73000 |
|
Emission maximum, nm: |
524 |
|
Fluorescence quantum yield: |
0.8 |
